EpiDiverse
TextbookEpidiverse ToolkitLectures
  • Introduction to Ecological Plant Epigenetics
  • Ecology
    • Phenotypic plasticity
      • Introduction: What is phenotypic plasticity?
      • Phenotypic plasticity at the molecular scale
      • Transgenerational plasticity and adaptation
      • Mechanisms of transgenerational responses
      • Ecological and evolutionary implications of phenotypic plasticity
      • References
    • Plant Defense Response
      • Priming
      • Abiotic factors
      • Biotic interactions
      • Transgenerational transmission of induced defenses
      • Future directions
      • Designing more ambitious studies
      • Conclusion
      • References
    • Epigenetics in Evolution
      • Current evolutionary theory
      • Extended Synthesis and future perspectives
      • Epigenetics role in evolution
      • Stability of epigentic marks
      • Phenotypic effects
      • Genetics - epigenetics
      • Natural patterns of DNA methylation
      • References
    • Genetic and epigenetic variation in natural populations across large spatial scales
      • Introduction: From genetic diversity to epigenetic diversity
      • Ecological levels of organization
      • Effects of Epigenetic Diversity
      • References
    • Conservation epigenetics
      • Conservation Epigenetics – will it come or will it go?
      • Increasing habitat and stress heterogeneity
      • Epimutation markers as a tool for conservation management
      • References
  • Molecular Biology
    • Chromatin organization and modifications regulating transcription
    • DNA Methylation
      • DNA methylation is the primary epigenetic mark
      • DNA methylation and demethylation
      • Distribution of methylcytosine in plant genomes
      • DNA methylation and imprinting
      • References
  • Bioinformatics
    • Bisulfite Sequencing Methods
      • Principles of Bisulfite Sequencing
      • Experimental Design
      • Library Preparation
      • Computational Processing
      • Alternative Methods
      • References
  • EpiDiverse Toolkit
    • Best Practice Pipelines
    • Installation
    • Troubleshooting
  • Lectures
    • Phenotypic plasticity - Vitek Latzel
    • Spatial patterns of epigenetic diversity - Katrin Heer
    • Natural variation of methylation - Detlef Weigel
  • Epigenetic talks
  • Appendix
    • Glossary
    • Acknowledgement
  • EpiDiverse
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  • Extra resources and getting help
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  1. EpiDiverse Toolkit

Troubleshooting

General troubleshooting guide for the EpiDiverse analysis pipelines

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Last updated 5 years ago

Singularity issues

Sometimes Singularity runs into problems when pulling multiple images at the same time for a pipeline run. In these instances it is sometimes better to pull the images manually into the directory that the pipeline will be run from, using for instance the following code:

singularity pull --name epidiverse-[PIPELINE]-[VERSION].simg \
docker://epidiverse/[PIPELINE]:[VERSION]

Check the for more information about configuring Singularity

Extra resources and getting help

If you still have an issue with running the pipeline then feel free to contact us at info@epidiverse.eu or by opening an issue in the respective pipeline repository on GitHub asking for help.

If you have problems that are directly related to Nextflow and not our pipelines then check out the or the .

Nextflow Documentation
Nextflow gitter channel
google group